Knowhow


In This Issue

Optimizing Your PC Hardware for DSV Recording Part I

Optimizing Your PC Hardware for DSV Recording Part II

TEM Sample Preparation of Irradiated GaN on a Sapphire Substrate

The importance of the specimen support film for cryo TEM

3View: A New Perspective for Cell Biologists

Phase identification and mapping based on valency loss EELS and ELNS

 


2007 Electron Microscopy Training Courses

 

SEMS 2007 WORKSHOP: TEM Digital Imaging Techniques for Bio-Medical

Introduction &Analysis (April 17-20, 2007)

EELS &Analysis (April 24-27, 2007)

TEM

SEM Specimen Preparation (May 3-4, 2007)

 

 

.Need-to-KNOW information on microscopy applications
Issue 15 April 2007

Optimizing Your PC Hardware for DSV Recording
Part I. Digital Streaming Video v.s. Analog Video

By Bill Mollon, Digital Imaging Product Manager, Gatan, Pleasanton, CA

In our December 2005 issue of KnowHow we introduced the DigitalMicrograph plug-in called Digital Streaming Video (DSV). The digital video stream as it is delivered by the DVCapture module within DM is intended to be used by another software program. Digital Micrograph merely acts as the supplier of the digital stream. It is up to the end-user to utilize other 3rd party mastering or authoring programs to capture and create “movies” from the stream. Movie creation in itself has many controllable variables that will affect the final output. Some are software related and others are highly dependent on the hardware being used. Namely, the PC and its components should be carefully considered when movie recording and creation is part of your application needs.

This article will go over the areas you should be aware of to optimize your hardware system for DSV recording.... Click here to read on.

 

Optimizing Your PC Hardware for DSV Recording
Part II. Recording Parameters Procedure (DigitalMicrograph)

Now that the hardware components have been identified and optimized you will need to make sure there is a proper “match” between the hardware and software settings within DigitalMicrograph. Use the following steps to setup your recording session: .... Click here to read on.

 

TEM Sample Preparation of Irradiated GaN on a Sapphire Substrate

By Prashanth Prasad, Materials Applications Engineer, Gatan, Pleasanton, CA

For much of the past decade, GaN has been a subject of extensive research due to very important technological applications of this material. Current applications of GaN include light-emitting diodes (LED's), laser diodes, UV detectors, and microwave power and ultra-high power switches. In the fabrication of such GaN-based devices, ion bombardment represents a very attractive tool for several technological steps, electrical and optical selective-area doping, dry etching, electrical isolation, quantum well intermixing, and ion-cut.

Successful applications of ion implantation depend on understanding the production and annealing of radiation damage. In this regard, transmission electron microscopy (TEM) plays a pivotal role. Consequently, preparation of good TEM samples is paramount in the detailed studies of ion implantation damage in GaN. Thus, knowledge of appropriate TEM sample preparation techniques is not only important for investigating fundamental defect processes in solids under ion bombardment but also essential for the fast developing GaN industry. ... Click here to read on.


The importance of the specimen support film for cryo TEM

By Linda Melanson, Life Science Applications Specialist, Gatan, Pleasanton, CA

An important factor that limits high resolution cryo TEM data collection is the preparation of the specimen. An important aspect of specimen preparation is the carbon support film to which the specimen is applied prior to plunge freezing in liquid ethane; surface charge, uniformity in thickness, planarity, and hole size and spacing all play a part in successful cryo preparation ... Click here to read on.



3View: A New Perspective for Cell Biologists

By Christel Genoud and Simon Galloway, Gatan UK

3View is a new product and technique from Gatan which is set to revolutionize microscopy in many fields. This is due to the relative ease with which high spatial resolution ultrastructure can be obtained from extensive volumes. The prototype for 3View (SBFSEM, serial block face imaging in the SEM) developed by Denk and Horstmann1 at MPI Heidelberg has pushed the spatial extent, and acquisition speed of 3D ultrastructure at relatively high spatial resolution. Furthermore, unlike other techniques the resolution in the Z direction can be close to the X,Y resolution, and it does not degrade with depth. As the technique is largely compatible with accepted EM specimen preparation for biological sciences, 3View is poised to transform microscopy in a variety of disciplines. ... Click here to read on.


 


Phase identification and mapping based on valence loss EELS and ELNES

By Mike Kundmann

 
Much of analytical TEM revolves around elemental analysis based on core-shell ionization and its role in electron energy-loss spectroscopy (EELS) and energy-dispersive X-ray spectroscopy (EDS). In these techniques, integrals of the primary or secondary ionization signals (typically over many tens of eV in energy) are used to measure and map the elemental composition of probed sample areas.. ... Click here to read on.


 

We hope that you find these articles to be interesting and informative. If you wish to subscribe to the Knowhow e-newsletter, simply click subscribe. Good luck in your research.

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Tel. (925) 463 0200 Fax. (925) 463 0204
Contact: info @gatan.com