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In This Issue
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Cryoplunge™3 Gatan’s new, affordable plunge freezing instrument
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How to optimize your EELS expirements by adjusting the collection angle of your spectrometer
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Are you worried about diffraction patterns damaging your CCD camera?
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Need-to-KNOW information on microscopy applications
Issue 19 December 2008
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Happy Holidays
As 2008 comes to a close and we celebrate the New Year, we would like to thank you for your amity and patronage. We hope that the application notes in the Knowhow and the introduction of new and improved products (e.g., ORIUS SC200D and SC200W cameras, Cryoplunge3, PIPS low energy milling and 3D Tomography GPU reconstruction software) have helped you achieve your research objectives. With your feedback, we will continue to design and improve our products to help you meet your application needs.
We wish you and your family the very best this holiday season and a happy New Year!
Ming Pan, Ph.D.
Director of Marketing
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Cryoplunge™3: Gatan’s versatile and affordable plunge-freezing instrument
By Linda Melanson, Gatan
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Cryoplunge3 (also known as Cp3) is Gatan’s new generation, semi-automatic plunge freezing instrument for the preparation of frozen hydrated specimens for cryo transmission electron microscopy (cryoEM.) The basic model for Cp3 consists of four major components: a support column, which houses all of the pneumatic logic and electrical components including the ethane temperature monitor, blotting timer and the humidity/temperature meter for monitoring the environmental conditions of the humidity chamber; a removable humidity chamber with blot assemblies, plunge piston, plunge tweezers, humidity/temperature sensor and humidity wand; a safety shield and interlocks to protect the user during the blotting and plunging cycle; and a specially designed, removable cryogenic workstation....................
To read the complete article click here
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How to optimize your EELS expirements by adjusting the collection angle of your spectrometer
By Alan Maigne, Gatan
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In EELS experiments, it is not possible to collect all the electrons scattered by the sample. Those limitations are mainly due to geometrical factors.
The spectrometer measures the number of electrons that have lost a specific amount of energy. The excitation of atoms in the sample will result in characteristic edges in the measured spectrum. The intensity of those edges is directly proportional to the number of atoms present and the scattering cross section of the studied element.
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The cross section is a function type of edge and depends strongly on the scattering angle. The fraction of signal collected for a particular transition (edge) varies as shown in Fig.2.
It’s critical to have a collection angle large enough to collect an important fraction of the desired scattered signal……………
To read the complete article click here
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Are you worried about diffraction patterns
damaging your CCD camera?
By Ming Pan and Bill Mollon, Gatan, Inc.
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If you ever attempted recording electron diffraction patterns with your CCD camera, does the diffraction pattern below (Figure 1) look familiar to you? Are you worried about the potential damage to the CCD sensor by the strong central spot in the diffraction pattern? Have you been warned never record any diffraction patterns with the CCD camera or you could permanently damage the CCD sensor?
Well, you do not have to worry about this anymore.
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The new ORIUS SC200D CCD camera is designed to allow users to record electron diffractions patterns without being worried about damaging the CCD and even more the camera can record diffraction patterns free from the common streaking artifact (figure 1). Figure 2a shows the SAED (selected area electron diffraction pattern) from LaCO3 crystal recorded by the SC200D CCD camera. Figure 2b shows the same pattern with contrast reversed. From these images it is clear that the streaking has been successfully eliminated....................
To read the complete article click here
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We hope that you will find this news letter both interesting and useful. If you do not, simply click here to be unsubscribed and removed from our list.
Corporate Headquarters, 5794 W. Las Positas Blvd. Pleasanton, CA 94588
Tel.  +1 (925) 463-0200, Fax. +1 (925) 463-0204
Contact: info @gatan.com
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