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Alto 2500 Cryotransfer for SEM
Cryotransfer for SEM allows imaging and analysis of biological and other hydrated, beam or vacuum sensitive specimens.
Specimen preparation techniques provided with every Alto system include rapid freezing (to preserve hydrated state), vacuum transfer (to prevent contamination), cold fracture (to expose internal microstructure), freeze etching (to clarify non aqueous components) and coating (to allow high resolution surface imaging and X-ray analysis).
Alto 2500 has been specifically designed to allow highest resolution performance together with Field Emission SEMs.
Specification of Alto 2500
- Nitrogen cold stage (-185°C to +50°C) and interface to SEM stage
- Anticontaminator and lamp assembly
- Nitrogen 'heat exchange' dewar and gas feedthroughs to SEM chamber
- Ball valve incorporating microswitches plus mechanical interlock and interface to SEM chamber
- High vacuum cryo preparation chamber, with integral nitrogen dewar, sample platform and variable temperature facility (-185°C to +100 C)
- Cold knife fracturing device
- Binocular viewer, x10 and x20 magnification
- Cold Magnetron sputter source for high resolution sputter coating (supplied with AuPd and Pt target)
- Airlock gate valve with interface for vacuum transfer device
- Turbomolecular pump system for cryo preparation chamber with 70 L/sec pump, high and low vacuum gauges
- Keypad control unit for vacuum, coating and temperature control, also system logic status
- Vacuum transfer device
- '2-pot' freezing station for producing nitrogen slush and loading pre-frozen samples
- Multi-functional two stage rotary pump, with exhaust filter, flexible pumping lines, foreline trap and ballast reservoir
- Power electronics and solenoid valves, mounted in floor standing cabinet
- Specimen holders including a range of stubs and rivets
- Self pressurising N2 dewar with gas flow regulator for cooling SEM cold stage and gas purging
- Spare parts kit
Options
- Carbon coater
- Additional specimen holders
- Rotary knife with micrometer control
- Quartz film thickness monitor
- Alternative coating targets, e.g. Pt, W and Cr (for microanalysis)
To download the Application Note, "Visualisation of Surfactants using Cryo-electron Microscopy" click here
To download the Application Note, "The use of cryo-SEM to Study nematodes', click here
To download the Application Note, Cryo techniques using low vacuum SEM', click here
To download the Application Note, "Observation of foods using cryo-SEM ', click here
To download the poster: "Low temperature scanning electron microscopy of cryo-planed and ion milled block faces", click here
To download the Application Note, "Investigations of Cementitious Materials with Low Temperature SEM', click here
To download the Application Note, "Cryo_SEM imaging with Gatan ALTO tilting, rotating sample holder', click here






