TEM Specimen Holders

CT3500 Single Tilt Liquid Nitrogen Cryo Transfer Holder

The CT3500 Single Tilt Liquid Nitrogen Cryo Transfer Holder is designed for low temperature transfer and subsequent recording of electron beam sensitive, frozen hydrated-specimens in the TEM.

Key Features

Frost-free specimen transfer

  • Cryo workstation provides low temperature specimen loading to protect the frozen-hydrated grid.
  • Shutter blades encapsulate the frozen-hydrated grid providing protection against damage caused by warming and frost formation during transfer from the workstation to the electron microscope.

High resolution performance

  • Stable specimen temperature minimizes thermal drift to ensure high resolution performance

Clamping ring specimen securing mechanism

  • Maintains excellent thermal contact between the specimen holder and the frozen-hydrated specimen grid

Optional beryllium specimen insert and Hexring®

  • For EDS analysis

Precision design

  • The specimen cradle that contains the frozen-hydrated grid is isolated from the holder tip.  Designed for the intermediate to expert electron cryomicroscopist.

Precise temperature measurement

  • Temperature of the holder is monitored by a calibrated thermocouple that provides a sensitive, linear temperature response
  • The conductor rod connecting the specimen holder tip to the liquid nitrogen dewar contains an electric heater to change the specimen temperature.

Tilt ranges and compatibility of specimen holders vary according to the TEM manufacturer, model, pole piece gap, and the presence of in-gap accessories.  Please contact your local Gatan representative for more information.  



Clamping ring screws into the specimen cradle




Two piece cryo shield protects the specimen during transfer

Training Resources:

  • CT3500 Training Video:
    • Click here for mp4 file
    • Click here for mwv file