Specimen Preparation

Cryoplunge™3

The Cryoplunge™3 (Cp3) is a semi-automatic plunge freezing instrument used for the preparation of frozen hydrated specimens for cryo TEM. (Application data) 

Key Features

 

Safe operation

  • Safety interlocks protect the user during the blotting and plunging cycle

 

Humidity chamber

  • Safe guards the specimen from drying during the blotting process
  • Humidity/Temperature metering of the humidity chamber
  • ~ 86% RH at the level of the specimen grid within ~15 minutes
  • Three entry points for specimen loading (right, left and front)

 

Specimen blotting

  • Removable blot pad assemblies
  • 1 or 2-sided blotting
  • Single and multiple blotting
  • Filter paper loading jig to minimize contact with filter paper while loading onto blot assemblies
  • Electronic blot timer accurate to 0.1 sec
  • Interchangeable blot assemblies
  • Adjustable blotting pressure
  • Any filter paper type can be used

 

Plunge and tweezer securing mechanism

  • Pneumatically assisted, multi-positional plunge rod
  • Quick disconnect tweezer mounting mechanism

 

Cryo workstation

  • Removable liquid nitrogen cryo workstation
  • Long hold, temperature monitored ethane vessel
  • Cryo grid box receptacle accepts round and square grid boxes
  • Filter paper blot stand within the pre-cooled workstation allows low temperature blotting of excess ethane for immediate viewing on TEM
  • External funnel for filling workstation with liquid nitrogen
  • Protective covers protect frozen hydrated grid during transfer from ethane to cryo grid storage box
  • Removable cryo grid box transfer vessel provides total protection of frozen hydrated grids during removal from workstation

 

Installation

  • Everything is included for fast, easy set-up
  • Light weight and portable with small footprint to minimize space demands

 

Cryoplunge™3 (Cp3)