3View Users Forum Agenda


Dinner will be provided for forum attendees who selected "Yes" to attending the dinner on their registration form.

Wednesday, 16 March 2016 at 19:00


Wirtshaus zum Seppl
Hauptstrasse 213, Heidelberg


Thursday, 17 March 2016


European Molecular Biology Laboratory (EMBL)
Large Operon Room, EMBL
Meyerhofstrasse 1
69117 Heidelberg

Bus schedule available for forum attendees.

Registration badges may be picked up at the following locations

  • 13 – 16 March: Registration desk of the EMBL workshop "From 3D Light to 3D Electron Microscopy"
  • 16 March: 3View User Forum Dinner
  • 17 March: Large Operon Room, EMBL

Forum Agenda

Session I: Chair Saskia Lippens, VIB Ghent
9:10 – 9:15 Welcome
Chris Guérin, VIB and Yannick Schwab, EMBL
9:15  9:45 Historical context of in-situ microtomy and serial block-face imaging by SEM
Alan Kuzirian, Woods Hole MBL
9:45  10:15 3D cartographic and quantitative analysis of large volume SBF-SEM data sets
Louise Hughes, Oxford Brookes University
10:15  10:45 3View – Different views of the process
Rick Webb, University of Queensland
10:45  11:15 Coffee break
11:15  11:35 Three-dimensional structure of chromosomes in a human prophase nucleus
Bo Chen, London Centre for Nanotechnology, UCL
11:35  11:55 CLEM: Current uses and future strategies
Nadine Soplop, The Rockefeller University
11:55  12:15 3D imaging of osteocytes using serial block-face scanning electron microscopy
Patricia Goggin, University of Southampton
12:15  12:35 3View spreading from life science to materials science
Armin Zankel, Graz University of Technology
12:35 – 13:15 Lunch
Session II: Chair Christel Genoud, Friedrich Miescher Institute
13:15  13:45 Haute-cuisine, or EM sample prep for 3View
Anneke Kremer, VIB Bio Imaging Core, Ghent
13:45  14:15
Optimisation of sample preparation for SBF-SEM of biomedical samples
Errin Johnson, Oxford University
14:15  14:35 3D structural analysis of cortical neuromodulatory circuits using correlated LM and SBF-SEM
Daniela Sahlender, EPFL
14:35  15:00 Preprocessing of SB-EM datasets using DigitalMicrograph and Microscopy Image Browser
Ilya Belevich, University of Helsinki
15:00  15:30 Advances in serial block-face imaging
Christopher Booth, Gatan
15:30  16:00 Coffee break
16:00  16:30 Best practices to enhance your serial block-face imaging results
Stuart Searle, Gatan
16:30  17:30 3View Users round table discussion (Q&A)
17:45 Travel to Heidelberg Centre


  • Yannick Schwab, EMBL
  • Nicole Schieber, EMBL
  • Chris Guerin, VIB Ghent