CEMAS 2021 Workshop: Advances in Direct Electron Detection
This virtual workshop is hosted by the Center for Electron Microscopy and Analysis (CEMAS), Ohio State University. Space is limited. For registration and program details, please visit https://cemas.osu.edu/events or contact Dr. David McComb.
January 20, 2021 (EST, USA)
- 1:00 – 2:00 p.m.: Cryo-EM presentation
- 2:00 – 4:00 p.m.: Discussion and Q&A
The direct electron detection technology has dramatically improved the quality of images for cryo-electron microscopy (cryo-EM), leading to the resolution revolution. With its exponential growth, the cryo-EM field continues to demand further improvement in data quality. Recently, the cryo-EM community has demonstrated that 200 keV transmission electron microscopes can equal the performance of 300 keV microscopes for macromolecular imaging. This has led to an era where having the highest imaging throughput and detective quantum efficiency of the detector becomes very crucial. Here we discuss, along with basics, some of the ongoing developments at Gatan, which can significantly impact the quality and efficiency of your cryo-EM data collection. The focus will be on electron counting and energy filtration, correlated double sampling mode, imaging throughput of the K3® camera, electron diffraction, and a brief introduction to the Latitude® software.
This CEMAS workshop will begin with a presentation on the principles and utilities of direct detection technology for cryo-EM. The workshop will finish with an open discussion and Q&A to confer on the technology, techniques, and applications presented. For more information on Gatan cryo-EM technology, please contact Bill Flosi.
Sahil Galuti, Ph.D., Gatan