In-situ

Real-time observation of growth processes, chemical reactions and oxidation, irradiation effects, mechanical, magnetic, and ferroelectric properties.

Overview Products Media Library Research Spotlight Publications Resources Back to top
Overview: 

What is in-situ microscopy?

In-situ transmission electron microscopy combines the image formation capabilities of the transmission electron microscope (TEM) with application of some external stimulus to measure results real-time. Currently, a wide variety of systems and holders are available to apply different stimuli to evaluate kinetic reactions such as catalysis, phase transformations, other chemical reactions, electrical fields, mechanical strain or nano-indentation deformation, heating and cooling, or the effects of the electron beam irradiation damage in samples. 

Historically, movies of various reactions and system kinetics could be recorded onto video tape. More recently screen capture programs allow low-resolution, qualitative capture for you to visualize what is happening at video frame rates, e.g., 30 frames per second (fps). This adds significant value to investigators who want to physically see and understand reactions in real-time.

With the advent of faster data transfer and processing capabilities, it is now possible to record and manage large datasets directly from the sensor output. You can store each pixel in each frame directly to disk, and treat them as an individual image. Alternatively you can apply various algorithms or scripts (e.g., summing, drift correction, binning, etc.) post-capture to quantify the stimulus applied to the system. This greatly enhances the flexibility of data management and gives you previously unseen resolution in time and space, with sub-ms time resolution to resolve reactions that previously were unknown.

                             

Video on the left taken with the OneView® IS camera, while video on the right was taken using the K2® IS camera.

Back to top