First 3.2 Å β-galactosidase structure solved by cryo-EM

First 3.2 Å β-galactosidase structure solved by cryo-EM

Structure of Escherichia coli β-galactosidase (∼465 kDa) at 3.2 Å resolution solved using the GIF Quantum LS energy filter that includes a K2 Summit camera. The first atomic model derived using single-particle cryo-EM analysis closely matches the 1.7 Å crystal structure with a global rmsd of ∼0.66 Å. Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11709-14.