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Getting started with your Elsa cryo-transfer holder

  1. Unpack and verify that the following parts are present:
    1. Elsa™ cryo-transfer holder (model 698)
    2. Temperature controller (model 1905)
    3. Cryo-transfer workstation (P/N 698.TFR)
    4. Valve upgrade kit (P/N 698.655UPG)
  2. If you are using an existing turbo pumping station (Model 655), install the valve upgrade kit (P/N 698.655UPG).
    1. Follow the instructions in 655.12000 Valve Installation Instructions.
Side-by-side view of valve replacement
   
  1. Place the temperature controller next to the turbo pumping station, ensuring adequate access to all connectors and controls.
  2. Connect the standard cable to the connector labeled Gatan 1 in the shown drawing:
Rear panel of temperature controller (model 1905)
   
  1. Connect the power cable to the temperature controller and plug into the wall.
  2. Turn on the temperature controller by briefly touching the button shown below:
Power button on temperature controller (model 1905)
   
  1. Insert the holder into the turbo pumping station.
  2. Connect the standard cable to the end of the Elsa holder (#1).
  3. Attach the pumping tube to the port on the Elsa holder (#2).
  4. Open valve #3, then open valve #4.
Cable and pumping tube connections plus valves on the Elsa holder
   
  1. Turn on the turbo pumping station.
  2. Using the menu on the temperature controller, set the system to Bake Out, then allow the system to bake out overnight.
  3. In the morning, first, close valve #4, then turn off the pumping station, and remove the holder for its first use.