Alpine Direct Detection Cameras

Models 1124, 1125

The world’s first direct detection cameras to democratize cryo-EM.

Advantages

Alpine^®is the world’s first direct detection camera series to democratize cryo-electron microscopy (cryo-EM) imaging. By enabling the acquisition of high-resolution structures on 80 – 200 keV microscopes, the Alpine series allows routine cryo-EM research on more affordable, workhorse instruments and expands its usefulness to the larger research community.

Two models available to match the field of view with your application needs:

Alpine (1124) – Turns 80 – 200 keV screening microscopes into data-collection microscopes with single-electron counting capabilities
Alpine Vista (1125) – Maximizes imaging throughput for your high-performance microscopes with a large FOV of 5,760 x 4,092 pixels

Delivers a detective quantum efficiency (DQE) up to 2.4-times the performance of scintillator-based cameras

Makes the 2D classification of small molecules possible while improving macromolecule reconstructions

Streamlines your workflow and detects failures early to produce better structures, faster

Real-time counting ensures there is no compromise between data collection speed and quality

Datasheet

Alpine Series

Media Gallery

Immerse yourself in the world of scientific discovery—explore striking microscopy images that reveal the unseen, alongside videos and tutorials showcasing Gatan technology in action.

Discover the Details

Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Apoferritin resolved to 2.7 Å at 100 keV

Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

When combined with Latitude software, Alpine delivers high-quality data at the highest imaging throughputs from 100 – 200 keV.

Transthyretin resolved to 4.6 Å at 100 keV

Transthyretin (55 kDa) resolved to 4.6 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) using CDS at ~5.2 e-/pixel/s. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.