OnPoint detector minimizes charging and beam damage

OnPoint detector minimizes charging and beam damage
Image courtesy of T. Deerinck NCMIR/UCSD.

Image shows that the OnPoint BSE detector can resolve features at 1.1 kV (right) that were previously distorted (e.g., black spots) by charging or beam damage at 2.2 kV (left). Sample: Mouse cerebellum. 

Preserves synaptic vesicle under low kV conditions

Synaptic vesicles
Image courtesy of T. Deerinck NCMIR/UCSD.

Demonstrates how low kV conditions can preserve and discriminate fine features, such as synaptic vesicles. Sample: 2 kV, 0.2 nm pixels, 24k x 24k image, 1 µs dwell time.

The Efficient Frontier of Resolution

The Efficient Frontier of Resolution

Comparison between the resolution and molecule size for published single-particle cryo-EM structures.

Clearly resolved myelin sheaths and collagen fibers

Clearly resolved myelin sheaths and collagen fibers

The zoomed in image (right) obtained using the OneView camera shows clearly-defined detail within the myelin sheathes and collagen fibers when compared to the original image (left).

Sample: skin; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

Fine details revealed in skin cells

Fine details revealed in skin cells

The zoomed in image (right) obtained using the OneView camera shows clearly-defined detail and resolution when compared to the original image (left).

Sample: skin; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

Clearly resolved collagen fibrils

Clearly resolved collagen fibrils

Collagen fibrils are clearly resolved in this 4k x 4k image obtained using the OneView camera. This definition is maintained when the original image is zoomed in on during post-processing.

Sample: collagen; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

High resolution image of cow intestine

High resolution image of cow intestine

OneView camera captures 4k x 4k resolution images of biological specimens, such as cow intestine.

High resolution lattice imaging of Au nanoparticles

High resolution lattice imaging of Au nanoparticles
Image courtesy JEOL Japan and Gatan, Inc., US

Because of OneView camera’s high resolution and high sensitivity, lattice imaging with a large field of view is effortless.

Sample: gold (Au) nanoparticles; beam energy: 300 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

HRTEM images of graphene

HRTEM images of graphene
Image courtesy JEOL Japan and Gatan, Inc., US

High sensitivity and drift correction of the OneView camera makes it easier to capture these HRTEM images of graphene.

Sample: graphene; beam energy: 80 kV; image size: 4k x 4k; exposure: 4 s; number of frames: 100

Drift correction compensates for brain sample motion

Drift correction compensates for brain sample motion

Sample was shrinking as it was exposed to the electron beam, these images were taken by the OneView camera before the objective aperture was inserted and specimen was stabilized.

Sample: brain; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25; drift correction: no (left) and yes (right)

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