Frozen-hydrated rotavirus double-layered particles

Frozen-hydrated rotavirus double-layered particles
Image courtesy of Dr. Debbie Kelly, Virginia Tech, Carilion

Methods

frozen-hydrated rotavirus double-layered particles (0.01 mg/mL concentration, 2 µL volume)
prepared on Affinity Grids decorated with His-tagged protein A and antibodies against the outer capsid protein, VP6
specimen prepared using Cryoplunge™ 3 instrument with GentleBlot™ technology on C-flat™ 2/1 grids, Protochips, Inc.
specimens examined under low dose conditions at 120 kV using a 626 liquid nitrogen cryo transfer holder
recorded at 50,000x, dose of  ~5 electrons/Å2

Frozen hydrated lipid vesicles

Frozen hydrated lipid vesicles
Image courtesy of Jessica Goodwin and Htet Khant, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX

Methods

prepared using Cryoplunge™ 3 instrument
image was recorded using UltraScan 4000 camera
TEM magnification of 25 kx at 200 keV
electron dose of ~20 e-2 
910 multi-specimen single tilt cryo transfer holder
sample prepared on Quantifoil specimen support
used Solarus advanced plasma cleaner prior to freezing

Frozen-hydrated image of the Ndc80 complex decorated microtubules

Frozen-hydrated image of the Ndc80 complex decorated microtubules
Image courtesy of Dr. Elizabeth Wilson‐Kubalek, Cell Biology, The Scripps Research Institute, La Jolla, CA

Methods

magnification 30kx
electron dose 40 e‐/Å2
specimens prepared on CFlat™ Protochips, Inc. CF‐2/1‐4C‐50 holey carbon grids
plasma cleaned using the Solarus® advanced plasma cleaning system
frozen hydrated preparations produced using Cryoplunge™ 3 instrument
image recorded at 100 kV on a TEM equipped with a model 626 70° single tilt liquid nitrogen cryo-transfer holder

Lattice images of gold particles deposited on a Quantifoil carbon film

Lattice images of gold particles deposited on a Quantifoil carbon film
Image courtesy of Dr. John Berriman and Dr. Christian Kübel at KIT, Institute of Nanotechnology, Karlsruhe, Germany

Lattice images of gold particles deposited on a Quantifoil® carbon film with grid held at -176 °C using the 914 high tilt liquid nitrogen cryo transfer tomography holder.

Methods

imaged using an UltraScan® 1000 2k x 2k camera
300 kV
8 s exposure
microscope magnification of 380 kx

Cryo-EM images of T7 phage

Cryo-EM images of T7 phage
Image courtesy of Dr. Zheng Liu, Markey Center for Structural Biology, Department of Biological Sciences, Purdue University

Grid was prepared using Cryoplunge™ 3 instrument. Image was recorded at 300 keV using 626 liquid nitrogen cryo-transfer holder.  
Scale bar 100 nm

Murano heating stage for EBSD studies

Murano heating stage for EBSD studies

Special compact design and heating method allows study of dynamic EBSD phase transformations within tight geometry restrictions of SEM.

Microtest 2000E replacement door and stage

Microtest 2000E replacement door and stage

Replacement SEM door and X,Y,Z stage with manual micrometer stage movement. Provides additional flexibility for some installations depending on SEM size and weight restrictions.

Microtest 2000E tensile stage

Microtest 2000E tensile stage

Dynamic testing module configured for in-situ EBSD studies.  Shown with optional EH2000 heated grips suitable for in-situ dynamic EBSD studies.

Atomic level EELS prepared in PIPS II system following FIB preparation (image 2)

Atomic level EELS prepared in PIPS II system following FIB preparation (image 2)
Data courtesy of Dr. Phil Rice and Dr. Teya Topuria, IBM, San Jose, CA

Fast atomic EELS analysis GaN/InGaN multi-layers sample preparation with PIPS II system and analysis with Gatan EELS product.

Atomic level EELS prepared in PIPS II system following FIB preparation

Atomic level EELS prepared in PIPS II system following FIB preparation
Data courtesy of Dr. Phil Rice and Dr. Teya Topuria, IBM, San Jose CA

Fast atomic EELS analysis GaN/InGaN multi-layers sample preparation with PIPS II system and analysis with Gatan EELS product.

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