Simultaneous EELS and EDS spectrum image
While advanced hardware can help any project, it was the vision, ingenuity and perseverance of the research team that made this remarkable achievement possible. Advanced publication is available online http://xlink.rsc.org/?doi=C4NR04553J. Nanoscale voxel spectroscopy by simultaneous EELS and EDS tomography; Haberfehlner, Orthacker, Albu, Lib and Kothleitner; Nanoscale, 2014, Advance Article
Graz and Leoben reconstruct spectral voxels from simultaneous EELS and EDS spectrum image tomography
Transmission electron microscopy (TEM) has long been limited to supplying only projections of three-dimensional objects limiting the information that can be obtained to two dimensions. Tilt series tomography provides 3D information, but for compositional tomography, the information is only qualitative.
Interplanetary dust particle HAADF
Data courtesy of Dr. Ilke Arslan of Sandia National Laboratories, Livermore, California and Dr. John P. Bradley of Lawrence Livermore National Laboratory, Livermore, California.
Interplanetary dust particle. STEM tilt series acquired with a 805 BF/ADF detector and 806 HAADF detector from -61° to 68°.
Interplanetary dust particle BF
Data courtesy of Dr. Ilke Arslan of Sandia National Laboratories, Livermore, California and Dr. John P. Bradley of Lawrence Livermore National Laboratory, Livermore, California
Interplanetary dust particle. STEM tilt series acquired with a 805 BF/ADF detector and 806 HAADF detector from -61º to 68º.
3D Tomo Carbon Nanotubes
Carbon nanotubes containing iron catalyst particles with TiO2 powder. EFTEM tilt series acquired with a Tridiem energy filter from -50º to 50º. A plasmon series (5 eV slit, 17.6 eV energy), titanium L-23 series (30 eV slit, 2 pre-edge, 1 post-edge), and iron L-23 series (40 eV slit, 2 pre-edge, 1 post-edge) were collected. Elemental series for titanium (blue) and iron (red) were generated with Gatan elemental mapping software and combined with the plasmon series (green).
K2 Summit camera and Quantum energy filter highlight the many forms of the MAC array also known as the bacterial death star
Shikuma, N. J.; Pilhofer, M.; Weiss, G. L.; Hadfield, M. G.; Jensen, G. J.; Newman, D. K.
Metamorphosis-associated contractile (MAC) structures show a surprising variety of uses in bacteria. Shown above is one particular structure which has been identified to be used in bacterial warfare. Data was collected using a GIF Quantum® energy filter with a K2 Summit® camera and is described by Shikuma et. al., in a recent issue of science.
Scale bar: 100 nm
Coalescence of nanoparticles
Courtesy of Litao Sun, Southeast University, Nanjing, China.
Gold nanoparticles on carbon film (Orius® 830 camera and 628 heating holder with SmartSet™ model 901 temperature controller on Tecnai 20). Movie was captured by In-situ video software.
In-situ nano-compression of a SiO2 glass nanoparticle
Courtesy of Zhiwei Shan at CAMP-Nano at Xi’an Jiaotong University, China.
A 500 nm amorphous SiO2 glass particle (top of the image) is plastically deformed into a pancake geometry in a compression test of Hysitron Picoindenter (PI95). The loading rate of 10 nm/s was generated by driving a flat diamond punch (lower left corner). Imaged with an Orius® 830 camera. Movie was captured by in-situ video software.
High tilt liquid nitrogen cryo-transfer tomography holder training, model 914
The 914 high tilt liquid nitrogen cryo -ransfer tomography holder is designed for low temperature transfer and subsequent tomographic recording of electron beam sensitive, frozen hydrated specimens in the TEM. Tilt ranges and compatibility of specimen holders vary according to the TEM manufacturer, model, pole piece gap, and the presence of in-gap accessories.
626 Single tilt liquid nitrogen cryo-transfer holder
The 626 single tilt liquid nitrogen cryo-transfer holder is designed for low temperature transfer and subsequent recording of electron beam sensitive, frozen hydrated specimens in the TEM. Tilt ranges and compatibility of specimen holders vary according to the TEM manufacturer, model, pole piece gap, and the presence of in-gap accessories.
Highly reproducible, high resolution Z sectioning
Data courtesy of Lucy Collinson, Cancer Research UK.
The high precision microtome allows section thicknesses to be cut at 15 nm, this allows isotropic resolution without the need to use deconvolution and multi-kV imaging methods, which can damage the same and reduce resolution.
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