Aldolase resolved to 3.07 Å at 100 keV

Aldolase resolved to 3.07 Å at 100 keV

Aldolase resolved to 3.07 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition.